Soluble Fibrinogen-Like Protein 2/Fibroleukin Exhibits Immunosuppressive Properties: Suppressing T Cell Proliferation and Inhibiting Maturation of Bone Marrow-Derived Dendritic Cells

摘要: Fibrinogen-like protein 2 (fgl2)/fibroleukin is a member of the fibrinogen-related superfamily. In addition to its established role in triggering thrombosis, it known be secreted by T cells. The soluble fgl2 ((s)fgl2) generated baculovirus expression system bound both cells and bone marrow-derived dendritic (DC) specific manner. (s)fgl2 exhibited immunomodulatory properties capable inhibiting cell proliferation stimulated alloantigens, anti-CD3/anti-CD28 mAbs, Con A dose-dependent manner; however, had no inhibitory effects on CTL activity. time- effect alloreactive could neutralized mAb against mouse fgl2. Polarization toward Th2 cytokine profile with decreased production IL-2 IFN-gamma increased IL-4 IL-10 was observed (s)fgl2-treated allogeneic cultures. Exposure immature DC abrogated CD80(high) MHC class II(high) molecules markedly inhibited NF-kappaB nuclear translocation, thus their maturation. (s)Fgl2-treated an impaired ability stimulate proliferation. Maximal inhibition when were cultured added culture. These data provide first evidence demonstrate that exerts immunosuppressive