Isolation and characterization of symbiotically defective pyrimidine and amino acid auxotroph of Mesorhizobium ciceri in chickpea.

摘要: Transposon Tn5 induced, four Mesorhizobium ciceri auxotroph were isolated and characterized. Unlike its wild type parent (TL 620), all mutants found defective for amino acid pyramiding biosynthesis. The characterized TL130 as cytosine uracil, TL 196 guanine, cytocine, uracil riboflavin, 141 serine 38 argentine defective. Symbiotic characterization of these revealed phenotypic deformities deficiencies in biological nitrogen fixation. All the auxotrophic nod+/fix+ nature with reduced nitrogenase activity 42.2, 26.3 17.13% respectively compared to which is further supported by sub cellular examination nodules section TEM study.