Sialylated core 1 based O-linked glycans enhance the growth rate of mammary carcinoma cells in MUC1 transgenic mice.

摘要: The MUC1 mucin, found on the luminal surface of simple epithelial cells is upregulated and aberrantly glycosylated in many carcinomas particularly breast ovarian. expressed by normal mammary cells, carries core 2 glycans but 1 based are added. binding monoclonal antibody SM3 to its peptide epitope tandem repeat blocked branched cells. Thus does not bind reacts with more than 90% carcinomas, suggesting that loss at least some a very common event carcinogenesis. To determine if change glycosylation observed confers an advantage cancer murine carcinoma cell lines were developed express carrying or linked glycans. vivo growth rate wild-type nude mice identical regardless O-glycosylation patterns. However, tumors grew out lost most their expression. In contrast, transgenic mice, where expression was retained tumor, striking difference observed. these expressing significantly faster These data suggest tolerant 2.