VARIATION IN THE LIFE-SPAN OF CLONES DERIVED FROM HUMAN DIPLOID CELL STRAINS

摘要: The doubling potential of several hundred clones derived from WI-38 and WI-26 cell cultures has been determined. Clones were isolated at various population levels (PDLs) during the finite in vitro life-span mass (uncloned) cultures. In all cases, there was a large variation (or life-span) among single culture. When which had undergone eight or nine doublings, only about 50% capable more than doublings. This percentage further reduced when higher PDLs. Mass appear to be composed two subpopulation classes: one with low potential, other potential. Nevertheless, highest observed any culture same as cells taken.