Human trophoblast-extracellular matrix (ECM) interactions in vitro: ECM thickness modulates morphology and proteolytic activity.

摘要: Trophoblast invasion of the uterine extracellular matrix, a critical process for human implantation and uteroplacental vascular development, is striking example controlled invasiveness. To examine cellular behavior relevant to this process, trophoblasts were cultured on (i) Millicell filters prelayered with Matrigel (ii) coverslips precoated gentle slope (Matribeach). Histologic sections system demonstrated significant invasion. However, Matribeach cells exhibited markedly different characteristics depending thickness Matrigel. On zone 1 (1-4 microns thick), flat aggregates syncytia seen. In contrast, 2 (4-14 microns) formed rounded intercellular processes. zone, prominent degradation pericellular proteins was assessed by both light microscopy scanning electron microscopy. Treatment 8-bromo-cAMP inhibited proteolytic process. 3 (14-60 microns), unicellular or small caused minimal matrix degradation. JEG-3 choriocarcinoma similar morphologic degradative properties Matribeach, but proteolysis not affected 8-bromo-cAMP. Our results suggest that has profound effects morphology activity. Furthermore, while normal malignant can degrade proteins, only trophoblast