Contractile and morphological properties of hamster retractor muscle following 16 h of cold preservation.

摘要: Introduction: Cold hypoxia is a common factor in cold tissue preservation and mammalian hibernation. The purpose of this study was to determine the effects on function retractor (RET) muscle hamster non-hibernating state compare these with previously published data (van der Heijden et al., 2000) [52] rat cutaneus trunci (CT) muscle. Materials methods: After storage (16 h at 4 degrees C). muscles were stimulated electrically measure maximum tetanus tension (P(0)) histologically analyzed. protective addition antioxidants trolox deferiprone calcium release inhibitor BDM fluid determined. Results: storage, twitch threshold current increased (from 60 500 mu A) P(0) decreased 27% control. RET morphology remained unaffected. protected by (P(0), resp., 43% 59% control). Addition had no effect RET. Conclusions: observed comparable those CT function, as reported [52]. Both show considerable functional damage due actions reactive oxygen species. In contrast CT, preservation-induced injury could not be prevented accompanied morphological such necrosis edema. This suggests that myocytes possess specific adaptation withstand Ca(2+) overload induced ischemia. (C) 2009 Elsevier Inc. All rights reserved.