Molecular Detection of Human Salivirus in Italy Through Monitoring of Urban Sewages
作者： Pamela Mancini , Giusy Bonanno Ferraro , Elisabetta Suffredini , Carolina Veneri , Marcello Iaconelli
DOI: 10.1007/S12560-019-09409-W
关键词:
摘要: Salivirus (SalV) is a newly discovered virus associated to acute gastroenteritis in humans. In Italy, its prevalence and genetic diversity unknown. To reduce this knowledge gap, 124 sewage samples collected throughout the country were analyzed for SalV by two nested RT-PCRs targeting 5′UTR 3D regions real-time RT-qPCR. Virus RNA was detected 37 (29.8%) samples; of these, 24 could be characterized all belonged genotype A1. Viral concentrations ranged between 2.8 × 103 1.9 × 105 genome copies per liter. This first report occurrence water environments suggesting that infection not uncommon country.
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