Iron specificity of the Fur-dependent regulation of the biosynthesis of the manganese-containing superoxide dismutase in Escherichia coli.

摘要: The Fur protein, which regulates iron uptake in Escherichia coli, also represses the biosynthesis of manganese-containing superoxide dismutase (MnSOD). A strain E. coli bearing a lacZ fusion to aerobactin operon was used compare metal specificities regulation MnSOD and by Fur. Iron, but not manganese, acted as corepressor Fur-dependent inhibition biosynthesis. iron-mediated dependent upon an intact fur locus, indicating that effect is mediated gene product. suppression accumulation iron, due destabilization polypeptide iron. Thus this seen sodA::lacZ production beta-galactosidase regulated sodA promoter. In contrast, both manganese served corepressors It thus appears effectiveness specific cations act with varies being Fur-metal complex.