Microbial transformation of polycyclic aromatic hydrocarbons in pristine and petroleum-contaminated sediments.

摘要: To determine rates of microbial transformation polycyclic aromatic hydrocarbons (PAH) in freshwater sediments, 14C-labeled PAH were incubated with samples from both pristine and petroleum-contaminated streams. Evolved 14CO2 was trapped KOH, unaltered polar metabolic intermediate fractions quantitated after sediment extraction column chromatography, bound cellular 14C measured residues. Large incorporated into material; therefore, measurement evolution alone would seriously underestimate [14C]naphthalene [14C]anthracene. compound turnover times increased 7.1 h for naphthalene to 400 anthracene, 10,000 benz(a)anthracene, more than 30,000 benz(a)pyrene. Turnover uncontaminated stream 10 greater contaminated samples, while absolute (micrograms per gram hour) 3,000 125,000 sediment. The data indicate that four- five-ring compounds, several which are carcinogenic, may persist even sediments have received chronic inputs support populations capable transforming two- three-ring compounds.