Beyond Field Effect: Analysis of Shrunken Centroids in Normal Esophageal Epithelia Detects Concomitant Esophageal Adenocarcinoma.

摘要: BACKGROUND AND AIMS: Because of the extremely low neoplastic progression rate in Barrett's esophagus, it is difficult to diagnose patients with concomitant adenocarcinoma early their disease course. If biomarkers existed normal squamous esophageal epithelium identify adenocarcinoma, potential applications would be far-reaching. The aim current study was global gene expression patterns capable revealing simultaneous even located remotely esophagus. METHODS: Tissues comprised epithelia from 9 8 lacking or Barrett's, and 6 esophagus alone. cDNA microarrays were performed, pattern recognition each these subgroups achieved using shrunken nearest centroid predictors. RESULTS: Our method accurately discriminated 8/8 without 6/6 alone 9/9 adenocarcinoma. Moreover, we identified genes differentially expressed between above subgroups. Thus, based on corresponding alone, our diagnosed who had CONCLUSIONS: These patterns, along individual culled them, represent for diagnosis epithelia. Genes discovered that are vs. merit further pursuit molecular genetic, functional, therapeutic interventional studies.