The role of desaturases in the biosynthesis of marking pheromones in bumblebee males
作者： Aleš Buček , Heiko Vogel , Petra Matoušková , Darina Prchalová , Petr Žáček
DOI: 10.1016/J.IBMB.2013.05.003
关键词:
摘要: Bumblebee males (Hymenoptera) produce species-specific labial gland secretions called marking pheromones (MPs). MPs generally consist of terpenoids and fatty-acid-derived aliphatic compounds with various chain lengths predominantly containing one or no double bonds. The unsaturated MP components were hypothesized to be produced by fatty acid desaturases (FADs) that exhibit diverse substrate specificities. To address this hypothesis, we isolated functionally characterized FADs from three bumblebee species: Bombus lucorum, terrestris, lapidarius. By employing RNA sequencing the male glands fat bodies B. lucorum identified five paralogous FAD-like sequences but only two FAD lineages abundant differentially expressed in glands. We found also body Functional characterization a yeast expression system confirmed Δ4-FADs exhibited unique Δ4-desaturase activity exclusively on 14-carbon acyls Δ9-FADs displayed Δ9-desaturase 14- 18-carbon acyls. These results indicate are involved biosynthesis major lapidarius despite composition these species. contribution lipases, acyltransferases, esterases, reductases production is discussed light transcriptomic data obtained study.
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