How to determine free Gd and free ligand in solution of Gd chelates. A technical note

摘要: Gd(III) chelates are often used as reporting probes in magnetic resonance imaging applications. Their use relies on the assumption that their high stability ensures against release of free ligand and Gd(3+) ions, which both highly toxic to living systems. The presence ions may have a profound effect contrast MR image they form hydrated, macromolecular complexes endowed with very relaxivity thus providing erroneous information extent induced by given probe. Although complex be enough, complexation step could not been completed. Therefore any Gd preparation has carefully checked for content metal ions. Herein currently procedures allow an assessment amounts solution described detail.