Prostaglandins as endogenous mediators of interleukin 1 production

摘要: We examined the role of cyclooxygenase (CO)-derived metabolites arachidonic acid (AA) in regulation interleukin 1 (IL 1) production by lipopolysaccharide (LPS)-stimulated murine resident peritoneal macrophages. The use LPS proved to be an efficacious probe, because it stimulated both IL and AA metabolism via only CO pathway. prostaglandin E2 (PGE2) I2 (PGI2; measured as its stable metabolite 6-Keto F1 alpha) LPS-stimulated macrophages was demonstrated high pressure liquid chromatography radioimmunoassay. addition exogenous PGE2 or PGI2 resulted a dose-dependent suppression macrophage production. Inhibitors pathway (indomethacin, piroxicam, ibuprofen) caused augmentation LPS-induced response. This directly correlated with efficacy compounds inhibitors. Similar results were found when macrophage-derived fibroblast growth factor assessed. cultures increase levels PGE2, over narrow dose range (0.05 0.6 units). These studies provide detailed evidence that synthesized can modulate factors In addition, our data support concept 1, classical hormones, regulate own through self-induced inhibitor, PGE2.