A molecular approach to provenance delineation for the restoration of hummock grasslands (Triodia spp.) in arid‐tropical Australia

摘要: Summary Studies were undertaken to develop genetic principles for the restoration of spinifex grasses (Triodia spp.) at Argyle Diamond Mines in northern Australia. The study used random amplified polymorphic DNA (RAPD) markers provide a molecular delineation provenance boundaries two species Curly Spinifex bitextura) and Triodia bynoei measures which ensure replacement local diversity. Within-population diversity (0.44 ± 0.03 T. bitextura 0.32 0.04 bynoei) proportion attributed between-population differences (≈ 12%) by analysis variance (AMOVA) indicate extensive out-crossing these seeder species. As such, both should be restored using seed-based methods maintain natural Significant groups populations (P < 0.05) identified AMOVA correspond directly clustering dendogram drawn UPGMA, indicating significant provenances For bitextura, genotypic variation 60 km from mine was not evident 30 km. Seed should, therefore, harvested within area maintenance provenance. Two located 2 apart found represent single genotype, all other as separate genotypes. majority isolated populations, distinct provenances. species, excess 80% measured occurred just 20 individuals. will necessitate harvesting seed thousands plants capacity evolutionary change maintained. With continuing improvements effectiveness genetics technology, assessment through use is becoming realistic option development more genetically representative sourcing genotypes plant programmes.