On the mechanism of the lycopene isomerase/cyclase reaction in Narcissus pseudonarcissus L. chromoplasts.
作者： P. Beyer , U. Kröncke , V. Nievelstein
DOI: 10.1016/S0021-9258(19)47341-9
关键词:
摘要: The lycopene cyclase reaction was investigated in Narcissus pseudonarcissus L. membranes using the terminal desaturation product of system, 7,9,9‘,7‘-tetra-cis-lycopene (prolycopene) as substrate. enzymatically formed bicyclic carotenes were trans configured, so overall consists two individual processes, isomerization and cyclization. reactions experimentally distinguished by use inhibitor [2-(p-chlorophenylthio)triethylammonium chloride. NADPH, essential cofactor for reaction, originates from plastid glycolytic pathway. It is shown that NADPH exhibits its function at stage also able to promote reduction prolycopene substrate vitro.
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