The metabolic pathway of flonicamid in oranges using an orthogonal approach based on high-resolution mass spectrometry and nuclear magnetic resonance

摘要: An orthogonal approach has been used to perform a metabolic profiling study of flonicamid in orange fruits. Nuclear magnetic resonance (NMR) and high-resolution mass spectrometry (HRMS) have applied monitor the degradation pathway into its metabolites field laboratory studies. 4-Trifluoromethylnicotinic acid (TFNA) 4-(trifluoromethyl)nicotinol glycine (TFNG) were detected after 15 days application studies, whereas trials, TFNG was found oranges 70 application. The results confirmed through use one-dimensional 1H, 2H 19F NMR, observing formation as well detection N-(4-trifluoromethylnicotinoyl)glycinamide (TFNG-AM) transient species, which is also known metabolite. deuteration methylenic carbon both observed, matter fact, exploited for first time route selective isotope labeling. Orthogonal techniques, such nuclear oranges. Thus, comprehensive view transformation process achieved taking advantage possibilities techniques.