Genomic studies and computational predictions of nucleosome positions and formation energies.

摘要: Chromatin is a complex of DNA, RNA, and proteins whose primary function to package genomic DNA into the tight confines cell nucleus. A fundamental repeating unit chromatin nucleosome, an octamer histone around which 147 base pairs are wound in almost two turns left-handed superhelix. dynamic structure that exerts profound influence on regulation gene expression other cellular functions. These chromatin-directed processes facilitated by optimizing nucleosome positions throughout genome remodeling nucleosomes response various external internal signals such as environmental perturbations. Here we discuss large-scale maps made available through recent advances parallel high-throughput sequencing microarray technologies. We show these reveal common features organization eukaryotic genomes. also survey computational models designed predict formation scores or energies demonstrate how predictions can be used position multiple without steric overlap.