Development of polymerase chain reaction primers highly specific for Xanthomonas albilineans, the causal bacterium of sugarcane leaf scald disease.

摘要: ABSTRACT New primers were developed that greatly improved the specificity of polymerase chain reaction (PCR) protocol for Xanthomonas albilineans, causal agent sugarcane leaf scald disease. Length-polymorphic PCR products, amplified under current from 16S-23S ribosomal DNA intergenic transcribed spacers (ITS) X. albilineans and three unidentified saprophytic bacterial species, cloned sequenced. Fourteen other nonredundant ITS sequences retrieved database highly homologous to sequence albilineans. Two albilineans-specific primers, namely, PGBL1 (5′ CTT TGG GTC TGT AGC TCA GG) PGBL2 GCC AGG TAT GC), designed based on a multiple alignment among these 18 sequences. These two permitted specific amplification 288-bp product all 71 diverse strains tested. No was observed any species tested, including un...