Cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of glyceraldehyde-3-phosphate dehydrogenase from Antheraea mylitta.
作者： Somnath Mukherjee , Samita Maity , Sobhan Roy , Suvankar Ghorai , Mrinmay Chakrabarti
DOI: 10.1107/S174430910903214X
关键词:
摘要: Glyceraldehyde-3-phosphate dehydrogenase from Antheraea mylitta (AmGAPDH) was cloned in pQE30 vector, overexpressed Escherichia coli M15 (pREP4) cells and purified to homogeneity. The protein crystallized using the hanging-drop vapour-diffusion method. crystals belonged orthorhombic space group I222, with unit-cell parameters a = 85.81, b 133.72, c = 220.37 A. X-ray diffraction data were collected processed maximum resolution of 2.2 A. presence three molecules asymmetric unit gave Matthews coefficient (V M) 2.80 A3 Da−1, solvent content 56.08%.
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