Remyelination of the Spinal Cord Following Intravenous Delivery of Bone Marrow Cells

摘要: Bone marrow contains a population of pluripotent cells that can differentiate into variety cell lineages, including neural cells. When injected directly the demyelinated spinal cord they elicit remyelination. Recent work has shown following systemic delivery bone functional improvement occurs in contusive injury and stroke models rat. We report here secondary to intravenous introduction an acutely isolated fraction (mononuclear fraction) from adult rat femoral bones separated on density gradient, ultrastructurally defined remyelination throughout focal lesion. The anatomical pattern was characteristic both oligodendrocyte Schwann myelination; conduction velocity improved remyelinated axons. were transfected express LacZ, β-galactosidase reaction product observed some myelin-forming cord. Intravenous injection other (Schwann olfactory ensheathing cells) or residual gradient did not result remyelination, suggesting specific mononuclear fraction. While precise mechanism repair, myelination by facilitation endogenous repair process, cannot be fully determined, results demonstrate unprecedented level myelin