Selective amplification system using Q-β replicase

摘要: Mutant forms of midivariant RNA are disclosed which replicated efficiently by the enzyme Qβ replicase in presence an agent(s) is useful for real time, quantitative detection accumulating products. This also suppresses replication non-mutant wild type RNA. The mutant MDV RNAs, when modified addition specific probe sequences, retain their properties agent. method allows to be monitored using a time mode thereby providing reliable monitoring kinetics reaction, and determining initial level amplified