Genetic Diversity of Arcobacter and Campylobacter on Broiler Carcasses during Processing

作者: INSOOK SON , MARK D. ENGLEN , MARK E. BERRANG , PAULA J. FEDORKA-CRAY , MARK A. HARRISON

DOI: 10.4315/0362-028X-69.5.1028

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摘要: Broiler carcasses (n = 325) were sampled at three sites along the processing line (prescalding, prechilling, and post-chilling) in a commercial poultry plant during five visits from August to October 2004. Pulsed-field gel electrophoresis (PFGE) was used determine genomic fingerprints of Campylobacter coli 27), jejuni 188), Arcobacter butzleri 138), cryaerophilus 1A 4), A. 1B 31) with restriction enzymes SmaI KpnI for Arcobacter, respectively. species subtyped by Centers Disease Control Prevention PulseNet 24-h standardized protocol C. jejuni. A modification this different endonuclease (KpnI) running conditions produced best separation fragment patterns species. Both unique common PFGE types strains identified. total of...

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