Nanotopographic Substrates of Poly (Methyl Methacrylate) Do Not Strongly Influence the Osteogenic Phenotype of Mesenchymal Stem Cells In Vitro

摘要: The chemical, mechanical, and topographical features of the extracellular matrix (ECM) have all been documented to influence cell adhesion, gene expression, migration, proliferation, differentiation. Topography plays a key role in architecture functionality various tissues vivo, thus raising possibility that topographic cues can be instructive when incorporated into biomaterials for regenerative applications. In literature, there are discrepancies regarding potential roles nanotopography enhance osteogenic phenotype mesenchymal stem cells (MSC). this study, we used thin film substrates poly(methyl methacrylate) (PMMA) with nanoscale gratings investigate on MSCs, focusing particular their ability produce mineral similar native bone. influenced focal adhesion size MSC alignment, enhanced proliferation after 14 days culture. However, was minimally by surface topography. Specifically, alkaline phosphatase (ALP) expression not increased nanotopographic films, nor calcium deposition improved 21 Ca: P ratios were mouse bone films 415 nm width 200 depth (G415) 303 190 (G303). Notably, surfaces had Ca∶P significantly lower than G415 films. Collectively, these data suggest that, PMMA nanogratings poor drivers an phenotype.