Cysteamine suppresses human peripheral blood mononuclear cells – human corneal endothelial cell reaction via reactive oxygen species reduction
作者： Joon Young Hyon , Won Ryang Wee , Soon Il Kwon , Young Joo Shin , Seonhowa Kim
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摘要: Purpose: To investigate the effect of cysteamine (CYS) on mixed peripheral blood mononuclear cells (PBMCs) - human corneal endothelial cell (HCEC) reaction (MLER). Methods: PBMC stimulation assay was performed using cultured HCEC. MLERs were treated with various concentrations CYS (0–20 mM). The proliferation rate and secretion profiles transforming growth factor-β1 (TGFβ1) interleukin-6 (IL-6) PBMCs stimulated by HCEC determined bromodeoxyuridine enzyme-linked immunosorbent assay, respectively. Results: suppressed in a dose-dependent manner (p<0.001). intracellular reactive oxygen species (ROS) levels decreased an increase concentration TGF-β1 IL-6 as well (p=0.011 0.003, respectively). Conclusions: This study showed that proliferation, via ROS formation. Our results suggest could suppress inflammation associated to cells.
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