Secondary structure model for bacterial 16S ribosomal RNA: phylogenetic, enzymatic and chemical evidence

摘要: We have derived a secondary structure model for 16S ribosomal RNA on the basis of comparative sequence analysis, chemical modification studies and nuclease susceptibility data. Nucleotide sequences E. coli B. brevis rRNA chains, RNAse T1 oligomer catalogs from rRNAs over 100 species eubacteria were used phylogenetic comparison. Chemical G by glyoxal, A m-chloroperbenzoic acid C bisulfite in naked rRNA, kethoxal active inactive 30S subunits was taken as an indication single stranded structure. Further support obtained to RNases T1. These three approaches are excellent agreement. The contains fifty helical elements organized into four major domains, which 46 percent nucleotides involved base pairing. Phylogenetic comparison shows that highly conserved found principally unpaired regions molecule. No knots created