Sensitivity comparison of PCR primers for detecting Wolbachia in spider mites.
作者： Xiao-Yue Hong , Tetsuo Gotoh , Hiroaki Noda
DOI: 10.1303/AEZ.2002.379
关键词:
摘要: Some primers occasionally fail to amplify Wolbachia DNA by a standard PCR from spider mites. In cross experiments, it is often necessary detect single individuals. Thus, six pairs of were evaluated for their sensitivity in detecting three mites, Panonychus mori Yokoyama, Oligonychus gotohi Ehara and Tetranychus kanzawai Kishida. Pure 100% infected populations these mites prepared, female adults the lines at day five examined detection using following primer pairs: wsp, 16S, ftsZ(Bf-Br), ftsZ(f1-r1), ftsZ(Adf-Adr) ftsZ(Z1-Z2). The wsp pair was most sensitive: detected even 1/1, 000 solution P. with 47.0% success rate. 16S second sensitive. ftsZ(Bf-Br) less They followed ftsZ(Z1-Z2) ftsZ(f1-r1) sensitivity. are suitable desirable use two simultaneously amplifying
sci-hub.se 参考文章(26)
Toru Nagata, Tetsuo Gotoh, Jun Sugasawa, Reproductive Compatibility of the Two-Spotted Spider Mite (Tetranychus urticae) Infected with Wolbachia Entomological Science. ,vol. 2, pp. 289- 295 ,(1999)
S. L. O'Neill, M. M. Pettigrew, S. P. Sinkins, H. R. Braig, T. G. Andreadis, R. B. Tesh, In vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line. Insect Molecular Biology. ,vol. 6, pp. 33- 39 ,(1997) , 10.1046/J.1365-2583.1997.00157.X
John H. Werren, Ary A. Hoffmann, Scott L. O'Neill, Influential passengers: inherited microorganisms and arthropod reproduction Oxford University Press. ,(1997)
Tetsuo Gotoh, Koh Gomi, Toru Nagata, Incompatibility and host plant differences among populations of Tetranychus kanzawai Kishida (Acari : Tetranychidae) Applied Entomology and Zoology. ,vol. 34, pp. 551- 561 ,(1999) , 10.1303/AEZ.34.551
Koh GOMI, Tetsuo GOTOH, Hiroaki NODA, Wolbachia Having No Effect on Reproductive Incompatibility in Tetranychus kanzawai KISHIDA (Acari: Tetranychidae) Applied Entomology and Zoology. ,vol. 32, pp. 485- 490 ,(1997) , 10.1303/AEZ.32.485
Tom T.M. Vandekerckhove, Stephanie Watteyne, Anne Willems, Jean G. Swings, Johan Mertens, Monique Gillis, Phylogenetic analysis of the 16S rDNA of the cytoplasmic bacterium Wolbachia from the novel host Folsomia candida (Hexapoda, Collembola) and its implications for wolbachial taxonomy. Fems Microbiology Letters. ,vol. 180, pp. 279- 286 ,(1999) , 10.1111/J.1574-6968.1999.TB08807.X
A. Tsagkarakou, T. Gulllemaud, F. Rousset, M. NavaJas, Molecular identification of a Wolbachia endosymbiont in a Tetranychus urticae strain (Acari: Tetranychidae). Insect Molecular Biology. ,vol. 5, pp. 217- 221 ,(1996) , 10.1111/J.1365-2583.1996.TB00057.X
Weiguo Zhou, François Rousset, Scott O'Neill, Phylogeny and PCR–based classification of Wolbachia strains using wsp gene sequences Proceedings of The Royal Society B: Biological Sciences. ,vol. 265, pp. 509- 515 ,(1998) , 10.1098/RSPB.1998.0324
Didier Bouchon, Thierry Rigaud, Pierre Juchault, Evidence for widespread Wolbachia infection in isopod crustaceans: molecular identification and host feminization Proceedings of The Royal Society B: Biological Sciences. ,vol. 265, pp. 1081- 1090 ,(1998) , 10.1098/RSPB.1998.0402
A. Jeyaprakash, M. A. Hoy, Long PCR improves Wolbachia DNA amplification: wsp sequences found in 76% of sixty‐three arthropod species Insect Molecular Biology. ,vol. 9, pp. 393- 405 ,(2000) , 10.1046/J.1365-2583.2000.00203.X