Brain-derived neurotrophic factor stimulates the neural differentiation of human umbilical cord blood-derived mesenchymal stem cells and survival of differentiated cells through MAPK/ERK and PI3K/Akt-dependent signaling pathways.
作者： Jung Yeon Lim , Sang In Park , Ji Hyeon Oh , Seong Muk Kim , Chang Hyun Jeong
DOI: 10.1002/JNR.21669
关键词:
摘要: Brain-derived neurotrophic factor (BDNF) plays an important role in the differentiation, development, and survival of neural stem cells. In this study, we analyzed its effects on stimulation human umbilical cord blood-derived mesenchymal cells terms their potential to differentiate into neuron-like cells, characteristics, molecular mechanisms involved. The treatment with induction medium (NIM) BDNF generated more that were produced stronger expression neural-lineage markers than treated NIM without BDNF. Raf-1 ERK phosphorylation p35 levels increased significantly both This also effectively blocked cell death following Akt Bcl2 compared Inhibition ERKs inhibited BDNF-stimulated up-regulation Bcl2. addition, inhibition PI3K abrogated expression, but not expression. Thus, MAPK/ERK-dependent PI3K/Akt-dependent contribute differentiation differentiated
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