Whole-Organ Tissue Engineering: Decellularization and Recellularization of Three-Dimensional Matrix Scaffolds

摘要: The definitive treatment for end-stage organ failure is orthotopic transplantation. However, the demand transplantation far exceeds number of available donor organs. A promising tissue-engineering/regenerative-medicine approach functional replacement has emerged in recent years. Decellularization organs such as heart, liver, and lung can provide an acellular, naturally occurring three-dimensional biologic scaffold material that then be seeded with selected cell populations. Preliminary studies animal models have provided encouraging results proof concept. significant challenges engineering remain. This manuscript describes fundamental concepts whole-organ engineering, including characterization extracellular matrix a scaffold, methods decellularization vascular organs, potential cells to reseed techniques recellularization process important aspects regarding biore...