Stimulation by follicle-stimulating hormone of DNA synthesis and of mitosis in cultured Sertoli cells prepared from testes of immature rats

摘要: Abstract Follicle-stimulating hormone (FSH) or dibutyryl 3',5'-cyclic AMP (dbcAMP) increases the incorporation of [ 3 H] thymidine into DNA cultured Sertoli cells prepared from testes rats 4 up to 40 days age. The amounts synthesis are shown be inversely related density cells, and maximal at periods 4–6 after plating. At a given cell extent by primary cultures unstimulatsd is greatest in preparations 10 age younger. percentage capable responding FSH dbcAMP with increased becomes progressively smaller older than 20 different stages development well correlated observed have labeled nuclei, as measured radioautographic examination, mitotic index. frequency mitosis presence FSH, younger rats. Evidence presented that which retain capacity undergo reduced nearly zero days. It concluded stimulation elicited directly associated an frequency.