Analysis of the env gene of a molecularly cloned and biologically active Moloney mink cell focus-forming proviral DNA.

摘要: A biologically active molecular clone of BALB/Moloney mink cell focus-forming (Mo-MCF) proviral DNA has been reconstructed in vitro. It contains the 59 half murine leukemia virus (Mo-MuLV) and 39 BALB/Mo-MCF DNA. The complete nucleotide sequence env gene long terminal repeat (LTR) cloned Mo-MCF determined compared with corresponding region parental Mo-MuLV substitution encompasses 1,159 base pairs, beginning carboxyl terminus pol extending to middle gene. product is predicted be 29 amino acids shorter than product. portion encoding p15E peptide identical both viral DNAs. There an additional residue a just preceding LTR. LTR similar that BALB/Mo-MuLV exception two single substitutions. We conclude responsible for dual-tropic properties viruses. Images