Planar cell polarity protein Dishevelled 3 (Dvl3) regulates ectoplasmic specialization (ES) dynamics in the testis through changes in cytoskeletal organization.
作者： Linxi Li , Baiping Mao , Ming Yan , Siwen Wu , Renshan Ge
DOI: 10.1038/S41419-019-1394-7
关键词:
摘要: In the mammalian testes, such as in rats, directional alignment of polarized elongating/elongated spermatids, particular step 17–19 across plane seminiferous epithelium resembles planar cell polarity (PCP) found hair cells cochlea. It is obvious that spermatid PCP necessary to support simultaneous development maximal number spermatids sustain daily production of > 50 million sperm per adult rat. Studies have shown testis indeed expresses multiple proteins PCP. Herein, using physiological and biochemical assays, morphological analysis, with technique RNA interference (RNAi) knockdown protein Dishevelled (Dvl) 1 (Dvl1), Dvl2, Dvl3, or Dvl1/2/3, Dvl proteins, it was Dvl3 played a crucial role Sertoli tight junction (TJ)-permeability barrier function through changes organization actin- microtubule (MT)-based cytoskeletons. More important, an vivo Dvl1/2/3 testis, defects were remarkably noted epithelium, concomitant adhesion transport, leading considerably spermatogenesis. triple also impeded MT-based cytoskeletons owing disruptive spatial expression MT-regulatory proteins. summary, particular, regulator blood–testis (BTB) its effects on cells.
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