Polyhydroxyalkanoate synthesis by mixed microbial consortia cultured on fermented dairy manure: Effect of aeration on process rates/yields and the associated microbial ecology.

摘要: Abstract Polyhydroxyalkanoates (PHAs) are biodegradable polymers that can substitute for petroleum-based plastics in a variety of applications. One avenue to commercial PHA production involves coupling waste-based synthesis with the use mixed microbial consortia (MMC). In this regard, requires maximizing enrichment MMC capable feast-famine synthesis, metabolic response induced through imposition aerobic-dynamic feeding (ADF) conditions. However, concept complex matrices remains unrefined; process operational improvements needed, along an enhanced understanding MMC. Research presented herein investigated effect aeration on four independent state systems studied; were fed volatile fatty acid (VFA)-rich fermented dairy manure. Regardless state, all exhibited based observed carbon cycling. Moreover, there was no statistical difference rates, qPHA ranging from 0.10 0.19 CmmolPHA gVSS−1 min−1; VFA uptake rates similar indifferences. assessments enriched resulted maximum intracellular concentrations 22.5 90.7% (mgPHA mgVSS−1); at concentration, mean hydroxyvalerate mol content 73 ± 0.6%. While typical dissolved oxygen (DO) pattern aeration, less resolution decreasing suggesting DO may not be optimal monitoring parameter. At lower states, nitrogen cycling patterns, supported by molecular investigations targeting AOBs and NOBs, indicate NO2 NO3 sustained synthesis. Next-generation sequencing analysis respective revealed numerous diverse genera exhibiting potential achieve functional redundancy embedded Ultimately, results demonstrate controlled ADF sustain potential, while enriching exhibits redundancy. Reduced could also enhance cost competitiveness production, further benefits associated treatment.