A monoclonal antibody-based immunosensor for the electrochemical detection of imidacloprid pesticide.
作者： Beatriz Pérez-Fernández , Josep V. Mercader , Brenda I. Checa-Orrego , Alfredo de la Escosura-Muñiz , Agustín Costa-García
DOI: 10.1039/C9AN00176J
关键词:
摘要: Imidacloprid (IMD) is one of the most used pesticides worldwide as a systemic insecticide well for pest control and seed treatment. The toxic potential carcinogenic character IMD makes its monitoring great relevance in field agriculture environment, so sensitive methodologies analysis are strongly required. In this context, we have developed competitive immunoassay determination using specific monoclonal antibodies followed by electrochemical detection on screen-printed carbon electrodes (SPCE). optimized immunosensor exhibited good reproducibility (RSD 9%) logarithmic response range 50–10 000 pM IMD, with an estimated limit (LOD) 24 pM, which was below maximum levels allowed legislation. High-Performance Liquid Chromatography-Mass Spectrometry-Mass Spectrometry (HPLC-MSMS) Enzyme-Linked Immunosorbent Assay (ELISA) were also performed comparison purposes, where wider lower limit. Matrix effects 6.5% obtained tap water samples. All these characteristics make our valid advantageous tool IMD.
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