Involvement of two latex-clearing proteins during rubber degradation and insights into the subsequent degradation pathway revealed by the genome sequence of Gordonia polyisoprenivorans strain VH2.

摘要: The increasing production of synthetic and natural poly(cis-1,4-isoprene) rubber leads to huge challenges in waste management. Only a few bacteria are known degrade rubber, little is about the mechanism microbial degradation. genome Gordonia polyisoprenivorans strain VH2, which one most effective rubber-degrading bacteria, was sequenced annotated elucidate degradation pathway other features this actinomycete. consists circular chromosome 5,669,805 bp plasmid 174,494 with average GC contents 67.0% 65.7%, respectively. It contains 5,110 putative protein-coding sequences, including many candidate genes responsible for biotechnically relevant pathways. Furthermore, we detected two homologues latex-clearing protein, supposed be key enzyme deletion these first time revealed clear evidence that protein essential utilization rubber. Based on sequence, predict supported by previous current data transposon mutagenesis, mutants, applied comparative genomics, literature search.