Molecular studies on Babesia, Theileria and Hepatozoon in southern Europe. Part II. Phylogenetic analysis and evolutionary history

摘要: Following a study on molecular epizootiology of Hepatozoon canis and piroplasmids (Babesia spp. Theileria spp.) in southern Europe, newly obtained sequences 18s rRNA gene were used for phylogenetic analysis. Partial analysed isolates showing high degree homology (>99%) with previous GenBank entries: H. canis, B. vogeli, equi (two isolates, Spain1 Spain2), T. annulata sp. The complete ovis bovis, that showed lower (<95%) rapport to previously reported species or isolates. A first set trees constructed partial most European clustered unambiguously described species, so minor sequence dissimilarities found are due probably strain variations. second was made using the 44 from sequenced bovis. analysis revealed time division five clades: (1) microti group, rodhaini, felis, leo, annae (proposed name without taxonomic value: Archaeopiroplasmids), (2) Western USA Theilerid-like group name: Prototheilerids), (3) containing all Bovinae Theilerids), (4) Babesia including gibsoni canids together divergens odocoilei Babesids), (5) composed mainly by ungulates: caballi, bigemina, ovis, bovis cow Ungulibabesids). bootstrap support several analytical procedures this new dicotomy Babesiidae always very high. Taking into account present additional paleogeographic, parasitological zoological evidences, two hypothesis origin evolution groups presented.