Anti-infective potential of hydroalcoholic extract of Punica granatum peel against gram-negative bacterial pathogens.

摘要: Background: Punica granatum extracts have been prescribed in traditional medicine for management of a variety disease conditions including microbial infections. Generation scientific evidence validation P. peel extract’s anti-pathogenic efficacy is required. Methods: Hydroalcoholic extract (PGPE), prepared by microwave assisted extraction method was evaluated its quorum-modulatory potential against two different human-pathogenic bacteria viz. Chromobacterium violaceum and Pseudomonas aeruginosa . Results: This able to modulate vitro production quorum sensing-regulated pigments both these test at ≥5 μg/ml. Virulence traits like haemolytic activity, biofilm formation were negatively affected the extract, it also made more susceptible lysis human serum. Antibiotic susceptibility modulated owing pre-treatment with PGPE. Exposure pathogens PGPE (≥0.5 μg/ml) effectively reduced their virulence towards nematode Caenorhabditis elegans Repeated subculturing on PGPE-supplemented growth medium did not induce resistance this notorious pathogen, found exert post-extract effect aeruginosa. Individual constituent phytocompounds be less efficacious than whole extract. seemed interfere signal-response machinery C. exhibited notable prebiotic promoting probiotic strains- Bifidobacterium bifidum Lactobacillus plantarum ≤50 Conclusions: study indicates an effective antipathogenic preparation, validates therapeutic use mentioned medicine. emphasizes need testing any bioactive broadest possible concentration range, particularly vivo , so that accurate picture dose-response relationship can emerge.