Metabolic characteristics of different malignant cancer cell lines.

摘要: Extracellular AMP inhibits cell proliferation of MCF- 7 and MDA-MB-453 whereas the highly malignant Novikoff line is not affected. In medium with low glucose supply cells grow well, are slightly inhibited MCF-7 totally unable to grow. Isoelectric focusing revealed that a glyclytic enzyme complex exists in all three lines. addition glycolytic enzymes, c-Raf-kinase, adenylate kinase, nucleoside diphosphate kinase also found within complex. The differences dependence lines can be explained by different constitutions shuttle enzymes. contain cytsolic glycerol 3-phosphate dehydrogenase which associated glyceraldehyde responsible for transport cytoslic hydrogen mitochondria. pI 7.8 form malate couples glycolysis glutaminolysis.