Bean class IV chitinase gene: structure, developmental expression and induction by heat stress

摘要: Abstract A P4-chitinase genomic sequence was isolated from a bean ( Phaseolus vulgaris ) library using P4-ch cDNA. The complete of the gene determined. Primer extension analysis allowed identification transcriptional start site located 79 bp upstream translational initiation codon. is interrupted by an intervening sequence. In 5′ region, TATT box occurs in place TATA at position −33, while typical CAAT found −33 with respect to transcription site. Gibberellic acid, heat shock and salicylic acid regulatory responsive sequences were also identified. Transient expression chimeric genes tobacco protoplasts indicated that all elements required for coding are present within first 600 flanking DNA. Various stress conditions such as wounding, NaCl treatments, cold have been applied plants. Whereas treatment ineffective, P4-mRNAs induced upon and, suprisingly, response stress. during germination seems be constitutively expressed roots mature