Effects of postprandial starvation on mRNA expression of endocrine-, amino acid and peptide transporter-, and metabolic enzyme-related genes in zebrafish ( Danio rerio )

摘要: The goal of this study was to systematically evaluate the molecular activities endocrine-, amino acid and peptide transporters-, metabolic enzyme-related genes in 35-day-old mixed-sex zebrafish (Danio rerio) after feeding . Zebrafish with initial body weights ranging from 9 11 mg were fasted for 384 h a controlled indoor environment. Fish sampled at 0, 3, 6, 12, 24, 48, 96, 192, fed. Overall, present results show that regulatory mechanism insulin-like growth factor I negative feedback regulated hormone is conserved zebrafish, as it mammals, but regulation receptors highly intricate. Leptin cholecystokinin are time-dependent signals, neuropeptide Y may be an important positive food intake zebrafish. acid/carnitine transporters B(0,+) (ATB(0,+)) broad neutral (0) transporter 1(B(0)AT1) mRNA levels measured our suggest protein utilized during 24-96 h fasting Glutamine synthetase downregulated, glutamate dehydrogenase, alanine aminotransferase, aspartate transaminase, trypsin upregulated longtime study. expression fatty decreased significantly (P < 0.05), whereas those lipoprotein lipase rapidly increased 96 h fasting. Fasting activated glucose synthesis when short periods time; prolonged, breakdown enzymes pentose phosphate shunt decreased.