Effect of Hangeshashinto on calprotectin expression in human oral epithelial cells

摘要: Oral epithelial cells produce antimicrobial peptides (AMPs) to prevent microbial infection. Calprotectin (S100A8/S100A9) is one of these AMPs in oral cells, the expression which up-regulated by interleukin-1α (IL-1α). Hangeshashinto (HST) a traditional Japanese herbal medicine that has anti-inflammatory effects. The purpose this study was investigate effect HST on calprotectin through regulation IL-1α cells. Human (TR146) were cultured with presence or absence anti-IL-1α antibody IL-1 receptor antagonist, six major components (3,4-dihydroxybenzaldehyde, baicalin, ginsenoside Rb1, glycyrrhizin, oleanolic acid and berberine). S100A8, S100A9, other cytokine mRNAs examined RT-PCR quantitative real-time PCR. secretion investigated ELISA. (6 μg/ml) increased S100A8/S100A9 protein, also β-defensin 2 (DEFB4) S100A7 expression. mRNA its protein slightly but significantly HST. A neutralizing against antagonist inhibited HST-up-regulated Although 3,4-dihydroxybenzaldehyde, baicalin Rb1 as expression, berberine decreased their These results suggest increases calprotectin, DEFB4 In response HST, up-regulation may be partially induced via IL-1α.