2-D DIGE as a quantitative tool for investigating the HUPO Brain Proteome Project mouse series.
作者： Melanie Föcking , Paul J. Boersema , Niaobh O'Donoghue , Gert Lubec , Stephen R. Pennington
关键词:
摘要: Brain development and aging is a complex process involving proliferation, differentiation apoptosis. Elucidating proteome changes in these processes can help to understand the mechanisms of brain maintenance as well neurodegenerative diseases. The research reported here contribution HUPO Proteome Project mouse pilot study. Whole, frozen C57BL/6J comprising three different developmental stages (embryonic day 16, postnatal 7, days 54-58) were processed by using 2-D DIGE. A total 1999 spots matched between all gels. Of these, 206 differentially expressed stages: 122 highest intensity embryonic stage E16, 26 juvenile group P7 58 P56, adult stage. results show pattern temporal expression. Based on expression patterns we tentatively suggest that proteins involved establishment primary structures are mouse. Proteins phase make utilization possible delivering energy mice.
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