An ultrasensitive immunochromatographic assay for non-pretreatment monitoring of chloramphenicol in raw milk
作者： Naifeng Xu , Liguang Xu , Wei Ma , Liqiang Liu , Hua Kuang
DOI: 10.1080/09540105.2014.998640
关键词:
摘要: A non-pretreatment monitoring method based on immunochromatographic test (ICT) strips was developed to for chloramphenicol (CAP) in raw milk. This assay designed measure competitive binding affinities CAP molecules milk and antigen immobilized with colloidal gold nanoparticles (GNPs, average diameter of 30 nm) labeled anti-CAP monoclonal antibody (CAP mAb). Several working conditions, including the solvent, different types concentrations mAb, coating antigen, GNP, were optimized performance. After optimization, detection process carried out 8 min a visual limit (LOD) 0.3 ng/mL qualitative LOD 0.064 semi-quantitative detection. No cross-reactivity detected toward analogs. Based these results, this simple ultrasensitive could be thoroughly applied on-site testing
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