NMR-based metabolomic analysis of wild, greenhouse, and in vitro regenerated shoots of Cymbopogon schoenanthus subsp. proximus with GC-MS assessment of proximadiol.

摘要: Cymbopogon schoenanthus subsp. proximus is a wild plant distributed in subtropical and east Africa extending from the north to southern parts of Egypt. Widely used folk medicine, it source diuretic sesquiterpene proximadiol. Nuclear magnetic resonance metabolomic analysis polar extracts shoots wild, greenhouse, somatic embryos, direct indirect organogenic vitro cultures was carried out. Metabolic profiling yielded 39 compounds, which common metabolites were 15 (38.4%). Unique trehalose (2.5%) plants, 2-hydroxylisobutyrate, galactarate tyrosine (7.6%) shoots. Tartrate found only regenerated (2.5%). Metabolites identified greenhouse embryogenic showed no unique compounds. Multivariate revealed significant differences between all tested 4-aminobutyrate, alanine, glutamine, glucose, fructose, sucrose most significantly different metabolites. Proximadiol quantitatively measured non-polar extract types using gas chromatography mass spectrometry (GC–MS). Concentrations ranged 3.6 ± 0.03 198.6 ± 7.2 µg/100 mg dry weight embryogenesis shoots, respectively. Direct organogenesis highest concentration (20.3 ± 0.5 µg/100 mg weight). This study reported metabolic C. primary that are type cultures. quantified culture system yielding relative identified.