Composition diversity and nutrition conditions for accumulation of polyhydroxyalkanoate (PHA) in a bacterial community from activated sludge
作者： Changli Liu , Haibiao Wang , Wenhui Xing , Li Wei
DOI: 10.1007/S00253-013-5165-6
关键词:
摘要: We investigated the effect of chemical oxygen demand (COD)/N ratio on polyhydroxybutyrate (PHB) accumulating ability in an anaerobic/aerobic cycle sequential batch reactor (SBR). Compared COD/N 10, 20, 50, and 125, 125 was most suitable nutritional proportion. When COD 1,200 mg/L COD/N/P 1,200/9.6/30, activated sludge PHB synthesis reached a maximum 64.2 % dry weight cells. The population detected periodically by denaturing gradient gel electrophoresis (DGGE). predominant strains belonged to five genera: Bacteroidetes sp., Acinetobacter Betaproteobacteria Gammaproteobacteria Arcobacter Bacillus sp. Pyrosequencing analysis 16S rRNA gene indicated that community more diverse than DGGE, specifically Acidobacteria (12.25 %), Alphaproteobacteria (10.78 Actinomycetales (9.68 Actinobacteria (5.15 Proteobacteria (4.04 unclassified bacteria (24.14 %).
springer.com
sci-hub.se 参考文章(43)
Leonardo Erijman, Eva L. M. Figuerola, Bacterial taxa abundance pattern in an industrial wastewater treatment system determined by the full rRNA cycle approach Environmental Microbiology. ,vol. 9, pp. 1780- 1789 ,(2007) , 10.1111/J.1462-2920.2007.01298.X
Guo-Qiang Chen, Plastics from bacteria : natural functions and applications Springer. ,(2010)
Xiaofen Wang, Shin Haruta, Pu Wang, Masaharu Ishii, Yasuo Igarashi, Zongjun Cui, Diversity of a stable enrichment culture which is useful for silage inoculant and its succession in alfalfa silage FEMS Microbiology Ecology. ,vol. 57, pp. 106- 115 ,(2006) , 10.1111/J.1574-6941.2006.00099.X
Andrew Eaton, A.E. Greenberg, Lenore S. Clesceri, Standard methods for the examination of water and wastewater Published in <b>1998</b> in Washington DC) by American public health association. ,(1992)
Bor-Yann Chen, Tz-Jau Shiau, Yu-Hong Wei, Wen-Ming Chen, Bing-Hua Yu, Chia-Yi Yen, Chung-Chuan Hsueh, Feasibility study of polyhydroxyalkanote production for materials recycling using naturally occurring pollutant degraders Journal of The Taiwan Institute of Chemical Engineers. ,vol. 43, pp. 455- 458 ,(2012) , 10.1016/J.JTICE.2011.08.005
Federico M. Ibarbalz, Eva L.M. Figuerola, Leonardo Erijman, Industrial activated sludge exhibit unique bacterial community composition at high taxonomic ranks Water Research. ,vol. 47, pp. 3854- 3864 ,(2013) , 10.1016/J.WATRES.2013.04.010
Salma Shahid, Ridha Mosrati, Jérôme Ledauphin, Caroline Amiel, Paul Fontaine, Jean-Luc Gaillard, David Corroler, Impact of carbon source and variable nitrogen conditions on bacterial biosynthesis of polyhydroxyalkanoates: Evidence of an atypical metabolism in Bacillus megaterium DSM 509 Journal of Bioscience and Bioengineering. ,vol. 116, pp. 302- 308 ,(2013) , 10.1016/J.JBIOSC.2013.02.017
F W Gilcreas, Standard methods for the examination of water and waste water. American Journal of Public Health and the Nations Health. ,vol. 56, pp. 387- 388 ,(1966) , 10.2105/AJPH.56.3.387
H. Chan, Removal and recycling of pollutants from Hong Kong restaurant wastewaters. Bioresource Technology. ,vol. 101, pp. 6859- 6867 ,(2010) , 10.1016/J.BIORTECH.2010.03.104
T. Z. DeSantis, P. Hugenholtz, N. Larsen, M. Rojas, E. L. Brodie, K. Keller, T. Huber, D. Dalevi, P. Hu, G. L. Andersen, Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB Applied and Environmental Microbiology. ,vol. 72, pp. 5069- 5072 ,(2006) , 10.1128/AEM.03006-05