An Eutherian-Specific microRNA Controls the Translation of Satb2 in a Model of Cortical Differentiation

摘要: Cerebral cortical development is controlled by key transcription factors that specify the neuronal identities in different layers. These are crucial for identity of neurons, but mechanisms controlling their expression distinct cells only partially known. Here we investigate and stability mRNAs Tbr1, Bcl11b, Fezf2, Satb2 Cux1 single developing mouse cells. We focus on find its mRNA occurs much earlier than protein synthesis a set broader expected, suggesting an initially tight control translation, which subsequently de-repressed at late developmental stages. Mechanistically, 39UTR modulates translation GFP reporters during corticogenesis. By vitro pull-down 39UTR-associated miRNAs, select putative miRNAs responsible SATB2 inhibition, focusing those strongly expressed early progenitor reduced miR-541, Eutherian-specific miRNA, miR-92a/b best candidates inactivation triggers robust premature both human Our findings indicate RNA interference plays major role timing cell may be part toolkit involved specifying supra-granular projection neurons.