A Proteomic Atlas of the African Swine Fever Virus Particle.

摘要: African swine fever virus (ASFV) is a large and complex DNA that causes highly lethal disease for which there no vaccine available. The ASFV particle, with an icosahedral multilayered structure, contains multiple polypeptides whose identity largely unknown. Here, we analyzed by mass spectroscopy the protein composition of purified extracellular particles performed immunoelectron microscopy to localize several detected proteins. proteomic analysis identified 68 viral proteins, account 39% genome coding capacity. proteome includes essentially all previously described virion proteins and, interestingly, 44 newly virus-packaged polypeptides, half have unknown function. A great proportion are committed architecture, including two structural p5 p8, derived from core polyproteins pp220 pp62, respectively. In addition, full complement enzymes factors involved in transcription, various implicated repair modification, some concerned entry host defense evasion. Finally, 21 many them localized at cell surface related cortical actin cytoskeleton, were reproducibly particle. Immunoelectron strongly supports suggestion these membrane-associated recruited during budding actin-dependent membrane protrusions. Altogether, results this study provide comprehensive model architecture integrates both compositional information.IMPORTANCE contagious currently affects countries sub-Saharan Africa, Caucasus, Russian Federation, Eastern Europe has very recently spread China. Despite extensive research, effective vaccines or antiviral strategies still lacking, basic questions on molecular mechanisms underlying infective cycle remain. One such gap regards structure infectious report, set compose determined inferred localization them. This information significantly increases our understanding biological features particle will help direct future research efforts.