Enhanced specificity of bacterial spore identification by oxidation and mass spectrometry.
DOI: 10.1002/RCM.1680
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摘要: Addition of an oxidizing agent (e.g., hydrogen peroxide) to intact spores selectively and completely oxidizes Met-containing biomarker proteins by formation Met sulfoxides. This reaction increases the masses observed in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) Bacillus Deltam = (16 x n) Da, where n is number residues sequence each individual protein. The procedure very rapid, can be performed situ (i.e., on MALDI target). It confirms identity biomarkers comparing amino acids from experimentally determined shifts with predictions for tentative acid In turn, accurate determination several allows rapid validation initial spore identification MALDI-MS.
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