Transcriptional Reprogramming of Xylem Cell Wall Biosynthesis in Tension Wood.
作者： Baoguang Liu , Juan Liu , Jing Yu , Zhifeng Wang , Yi Sun
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摘要: Tension wood (TW) is a specialized xylem tissue developed under mechanical/tension stress in angiosperm trees. TW development involves transregulation of secondary cell wall genes, which leads to altered properties for adaptation. We induced the stems California poplar (Populus trichocarpa, Nisqually-1) and identified two significantly repressed transcription factor (TF) genes: class B3 Heat-Shock Transcription Factor (HSFB3-1) MYB092. Transcriptomic analysis chromatin immunoprecipitation (ChIP) was used identify direct TF-DNA interactions P. trichocarpa protoplasts overexpressing TFs. This established transcriptional regulatory network PtrHSFB3-1 PtrMYB092 directly activate 8 11 monolignol respectively. The were verified their specificity transactivator roles 35 independent CRISPR-based biallelic mutants overexpression transgenic lines trichocarpa. gene-edited trees (mimicking tension stress) have stem composition resembling that during normal growth (i.e., low lignin high cellulose), whereas overexpressors showed an opposite effect (high cellulose). Individual TFs impeded reduction restored levels biosynthesis TW. study offers biological insights further uncover how metabolism, growth, adaptation are coordinately regulated
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