Single chain fragment variable (scFv) antibodies targeting the spike protein of porcine epidemic diarrhea virus provide protection against viral infection in piglets

摘要: Porcine epidemic diarrhea virus (PEDV) is a highly contagious coronavirus that causes severe and death in neonatal piglets. Passive immunization with neutralizing antibodies against PEDV an effective prevention measure. In this study, single chain fragment variable (scFv) were screened from the porcine scFv phage display library. After four rounds of biopanning, scFvs showed higher affinity to antigen selected for further study. The genes cloned into expression plasmid recombinant protein expression. These shown inhibit infectivity by plaque reduction neutralization assay. Immunofluorescence assay (IFA) revealed epitopes recognized these S1 region spike protein. potential provide infections piglets was investigated. Piglets orally administered no mild clinical symptoms, significantly less viral shedding, mortality intestinal lesions. field application also survival rate increased oral administration scFvs. Our data support role treatment infection.