Innate Immunity in the Human Female Reproductive Tract: Antiviral Response of Uterine Epithelial Cells to the TLR3 Agonist Poly(I:C)
作者： Todd M. Schaefer , John V. Fahey , Jacqueline A. Wright , Charles R. Wira
DOI: 10.4049/JIMMUNOL.174.2.992
关键词:
摘要: The objective of this study was to examine the expression TLR by human primary uterine epithelial cells (UEC) and determine whether exposure agonist poly(I:C) would induce an antiviral response. secretion several cytokines chemokines examined as well mRNA beta-defensin-1 -2 (HBD1 HBD2), IFN-beta, IFN-beta-stimulated genes myxovirus resistance gene 1 2',5' oligoadenylate synthetase. TLR1-9 UEC demonstrated RT-PCR, with only TLR10 not expressed. Stimulation TLR3 induced proinflammatory TNF-alpha, IL-6, GM-CSF, G-CSF, CXCL8/IL-8, CCL2/MCP-1, CCL4/MIP-1beta. In addition, HBD1 HBD2 6- 4-fold, respectively. Furthermore, upon initiated a potent response resulting in induction IFN-beta 70-fold synthetase (107- 96-fold), These results suggest that line cavity are sensitive viral infection and/or dsRNA released from killed cells. Not do release mediate initiation inflammatory recruitment immune site infection, but they also express beta-defensins, can have direct inhibiting effect on replication.
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